06/14/2021
Challenges in development of robust analytical methods for the quantitation of low molecular weight compounds by LC-MS/MS
Method development can be considered one of the most challenging task in a Bioanalytical laboratory. When working with LC-MS/MS and low molecular weight drugs, low degree of ionization, poor fragmentation, higher presence of isobaric compounds or analyte loss due to their high volatility can get in the way of a successful method development.
In the work we presented during the 7th Young Scientist Symposium, we aim to provide ideas and strategies to help tackle and solve these problems, based on real issues encountered during method development.
The advice given in the poster when working with low mass compounds focuses on three main points:
• Evaluate alternative ionization sources in order to potentially reduce background noise and increase overall sensitivity.
• Carefully evaluate and investigate the evaporation step in order to reduce cross-contamination, even avoid it altogether when possible.
• Higher presence of endogenous isobaric compounds, that can have the same MRM transition, means that the chromatographic separation has to be extensively optimized. Good chromatographic separation of all isobaric compounds is paramount to avoid erroneous conclusions.
You may find the poster below for your ready reference:
MORE NEWS
Peptides Series: Understanding Therapeutic Peptides
Therapeutic peptides are transforming modern medicine thanks to their unique advantages over traditional drug therapies. Their molecular structure and biological properties allow them to achieve effects like natural molecules in the body, enabling targeted action with fewer risks of off-target effects.
The visit of the Mayor of Barcelona, Jaume Collboni, to Anapharm Bioanalytics
Last Friday, January 10th 2025, we had the honor of welcoming the Mayor of Barcelona, Jaume Collboni, to our facilities. His visit was a special opportunity for us to showcase the work we do at Anapharm Bioanalytics and to reaffirm our commitment to innovation in the field of bioanalysis.
Overcoming Matrix Effects in the Analysis of Lipophilic Compounds
At the 10th EBF Young Scientist Symposium, we highlighted the complexities of analysing Fucoxanthin derivatives and demonstrated how innovative approaches, such as 2D Chromatography, can overcome these obstacles.