12/17/2017
New LC-MS/MS bioanalytical method for quantification of Estradiol and Estrone
A new method has been successfully developed and validated for determination of Unconjugated Estradiol and Estrone in human plasma by LC-MS/MS with LLOQ of 2 pg/mL and 5 pg/mL respectively.
The method involves a liquid-liquid extraction procedure and a subsequent derivatization procedure. Estradiol, Estrone and internal standards are measured by reversed phase ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS).
The possible metabolite back conversion has been evaluated during method validation.
This method has been successfully used to analyze samples from different clinical studies and the incurred sample reproducibility has met all acceptance crieria.
The development of this assay adds to the list of high sensitivity methods at Anapharm together with Tiotropium, Fluticasone, Salmeterol, Formoterol or Ethinyl Estradiol.
MORE NEWS
Overcoming Matrix Effects in the Analysis of Lipophilic Compounds
At the 10th EBF Young Scientist Symposium, we highlighted the complexities of analysing Fucoxanthin derivatives and demonstrated how innovative approaches, such as 2D Chromatography, can overcome these obstacles.
Overcoming Analytical Challenges in Liraglutide & Semaglutide Analysis
The work presented at the 10th EBF Young Scientist Symposium highlights the analytical challenges of developing UPLC/MS/MS methodologies for the extraction and quantification of Liraglutide and Semaglutide in plasma, while also outlining the strategies employed to solve them.
Anapharm Bioanalytics Recognized as an Innovative SME
We are thrilled to announce that Anapharm Bioanalytics has recently been officially recognized as an Innovative SME (PYME Innovadora) by the Spanish Ministry of Science and Innovation.